New Approaches to Molecular Profiling of Tissue Samples
نویسندگان
چکیده
Carolyn J.M. Best a, John W. Gillespie a,c, Chad R. Englert a, Jennifer I. Swalwell a, John Pfeifer b, David B. Krizman c,d, Emanuel F. Petricoin e, Lance A. Liotta d and Michael R. Emmert-Buck a,∗ a Pathogenetics Unit, Laboratory of Pathology, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA b Center for Information Technology, National Institutes of Health, Bethesda, MD, USA c Cancer Genome Anatomy Project, Advanced Technology Center, National Cancer Institute, Gaithersburg, MD, USA d Office of the Chief, Laboratory of Pathology, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA e Division of Cytokine Biology, Center for Biologics and Research, Food and Drug Administration, Bethesda, MD, USA
منابع مشابه
Proteomics Profiling of Chimeric-Truncated Tissue Plasminogen activator Producing- Chinese Hamster Ovary Cells Cultivated in a Chemically Defined Medium Supplemented with Protein Hydrolysates
Background: Culture media enrichment through the addition of protein hydrolysates is beneficial for achieving higher protein expression. Methods: In this study, designing the optimum mixture of four soy and casein-derived hydrolysates was successfully performed by design of experiment and specific productivity increased in all predicted combinations. Protein profile of recombinant CHO (rCHO) ce...
متن کاملRNA Extraction from Animal and Human\'s Cancerous Tissues: Does Tissue Matter?
The reliability of gene expression profiling based technologies and methods to find transcriptional differences representative for the original samples is influenced by the quality of the extracted RNA. Hence, RNA extraction is the first step to investigate the gene expression and its function. Consequently, the quality of extracted RNA is really significant. Correspondingly, this research was ...
متن کاملThe significance of gene profiling in diagnosis the cause of drug resistance in cancer
Chemoresistance is one of the main obstacles to the success of cancer treatment and one of the most important causes of death in patients. In the last decade, progress in high-throughput technologies, including microarray, sequencing, and bioinformatics has greatly resulted in cancer gene profiling and identification of biomarkers for cancer prognosis and prediction. This has greatly improved t...
متن کاملRNAi technology: A Novel approaches against fungal infections
Despite the introduction of new antifungal agents, resistances to antifungal therapy continue to increase and outcome of invasive fungal infections treatment is frequently suboptimal. A large amount of the recent effort in antifungal drug discovery has focused on a limited set of targets with functions known or expected to be important for fungal viability and virulence. A variety of techniques...
متن کاملA Document Weighted Approach for Gender and Age Prediction Based on Term Weight Measure
Author profiling is a text classification technique, which is used to predict the profiles of unknown text by analyzing their writing styles. Author profiles are the characteristics of the authors like gender, age, nativity language, country and educational background. The existing approaches for Author Profiling suffered from problems like high dimensionality of features and fail to capture th...
متن کاملNon-laser capture microscopy approach for the microdissection of discrete mouse brain regions for total RNA isolation and downstream next-generation sequencing and gene expression profiling.
As technological platforms, approaches such as next-generation sequencing, microarray, and qRT-PCR have great promise for expanding our understanding of the breadth of molecular regulation. Newer approaches such as high-resolution RNA sequencing (RNA-Seq)(1) provides new and expansive information about tissue- or state-specific expression such as relative transcript levels, alternative splicin...
متن کامل